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QX200 ddPCR System w_Laptop (BIO-RAD)

QX200 ddPCR System w_Laptop

  • Disponibilidad:
    • Cod. Prov: 1864001
    • -
    • Ref. Teknovas: D61864001
    • -
    • Marca: BIO-RAD

Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications.

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Descripción

Descripción & Documentación

The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.

Benefits of the QX200 Droplet Digital PCR System

  • Most precise and sensitive digital PCR solution for a wide variety of applications
  • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Flexible assay setup — scalable for high sensitivity or high throughput
  • Simple and easy-to-use workflow with 96-sample throughput
  • Droplet partitioning by the QX200 Droplet Digital technology reduces bias from amplification efficiency and PCR inhibitors
  • Convenient assay design — standard curves are not required

See performance data for the QX200 Droplet Digital PCR System.

Applications of the QX200 Droplet Digital PCR System

  • Cancer biomarker studies and copy number variation
    Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. 
  • Pathogen detection
    Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring.
  • Next generation sequencing
    Perform accurate quantification and qualification of NGS libraries without the use of a standard curve.
  • Gene expression analysis
    Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA.
  • Environmental monitoring
    Test a wide variety of environmental samples like soil and water using the QX200 System.
  • Food testing
    Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.

Droplet Digital PCR Technology and Workflow

The QX200 Droplet Digital PCR System consists of two instruments, the QX200 Droplet Generator and the QX200 Droplet Reader, and their associated consumables.

The QX200 Droplet Generator is used to partition ddPCR reaction mix into thousands of nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are analyzed individually on the QX200 Droplet Reader. Droplets are read well by well as they are streamed single file past a two-color optical detection system in a serial manner. Up to 96 samples can be processed per run. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. Alternatively, amplified products can be extracted from droplets following PCR for downstream applications, such as sequencing or cloning.

Additional Instruments

The QX200 Droplet Digital PCR System requires the use of the C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module and the PX1 PCR Plate Sealer.

Required Accessories

    • Referencia:
    • D61864001
    • Especificaciones:
    • Starting sample size, µl 20
      QX200 droplet generator capacity
      1–8 samples/cartridge
      Droplets per 20 µl sample 20,000
      QX200 droplet reader capacity
      1–96 samples
      Sample illumination
      Light-emitting diodes
      Sample detection
      Multi-pixel photon counter
      Detection channels
      FAM (EvaGreen), HEX (VIC)
      Linear dynamic range
      5 orders of magnitude
      Precision
      ±10%
      Droplets per 96-well plate, million
      ~1.5

      QX200 droplet generator dimensions

      (W x D x H)

      28 x 36 x 13 cm
      (11 x 14 x 5")
      QX200 droplet reader dimensions
      (W x D x H)
      66 x 52 x 29 cm
      (26 x 20 x 11")
    • Categoría de Producto:
    • Biología Molecular
    • Subcategoría de Producto:
    • Equipos PCR Digital
    • Disponibilidad:
    • Disponible en 10 días laborables
    • Aplicaciones:
    • Amplificación (PCR y qPCR)