Kit de transfección de células primarias para Nucleofector 4D, unidad Y-AD1, AD kit, 1 kit de 24 reacciones

Electroporation-based methods have so far required cells to be in suspension for transfection. However, the 4D-NucleofectorTM Y Unit allows direct Nucleofection of cells in adherence. Cells which typically grow in adherence in cell culture, can be kept and transfected by Nucleofection in their physiological state. 

The AD1 Primary Cell 4D-NucleofectorTM Y Kit is one of our two kits that are suited for electroporation of primary cells in adherence when working with the 4D-NucleofectorTM Y Unit. The cells are cultured in the 24-well culture plates provided with the kit* and for the transfection step a 24-well Dipping Electrode Array is inserted after replacing culture medium by the specific NucleofectorTM Solution mixed with the DNA or RNA substrate.

This kit has been determined to be optimal for transfecting following cell types:

• AoSMC (rat)
• Dorsal root ganglia DRG (rat)
• HUVEC (endothelial cells, umbilical vein, human)
• Neurons (mouse), cortex
• Neurons (rat), cortex
• Neurons (rat), hippocampal
• Osteoblast (rat)
• PAEC (endothelial cells, pulmonary artery, human)
• Retinal cells (rat)

Benefits

• Nucleofection of neuronal cells at any time point during their culture period, i.e. at a later developmental stages
• Nucleofection of cells in adherence without the need for transfer in suspension
• Transfection efficiencies up to 70% combined with high viabilities