UltraScript 2.0 cDNA Synthesis Kit cDNA Synthesis

UltraScript 2.0 Reverse Transcriptase is a robust and highly thermostable modified MMLV reverse transcriptase engineered for superior cDNA synthesis speed, yield and representation from a wide range of RNA sample types, including GC-rich and low abundance templates. The enhanced thermostability of UltraScript 2.0 Reverse Transcriptase (RTase) allows reaction temperatures of over 55°C to be used, giving improved specificity, higher cDNA yields, and more full length cDNA product. 

For RT-qPCR applications, the 2-tube UltraScript 2.0 cDNA Synthesis Kit contains all the components required for fast, reliable and unbiased cDNA synthesis, including an optimised blend of anchored oligo(dT) and random hexamers. UltraScript 2.0 cDNA Synthesis Kit Separate Oligos provides anchored oligo(dT) and random hexamers in separate tubes, for user optimisation depending on the type of analysis needed. 

UltraScript 2.0 kits can be used with 20pg to 3.5μg total RNA or oligo(dT) purified mRNA. The RTase is pre-blended with an advanced RNase inhibitor to prevent degradation of RNA by contaminating RNase.