TRIPZ shRNA Packaging Starter Kit (glycerol kit)
Disponible
Study the function of essential genes, validate cellular phenotypes and generate inducible knockdown cell lines with TRIPZ Inducible Lentiviral shRNA.
Aplicaciones | Silenciamiento génico |
---|---|
Categoría de Producto | Empaquetamiento viral |
Temperatura Envío | Congelado -20º |
Temperatura Almacenaje | Congelado -80º |
Disponibilidad | Bajo pedido |
Especificaciones | Tet-inducible shRNA expression permits reversible and regulatable gene knockdown microRNA-adapted shRNA design enhances specificity and potency of silencing TurboRFP fluorescent reporter allows visual tracking of shRNA expression Lentiviral vector enables transductions of primary and non-dividing cells Tet-On or Tet-Off configurations The pre-designed TRIPZ shRNA collection is configured for expression of an shRNA in the presence of doxycycline (Tet-On configuration). However, it is possible to alter the construct such that expression occurs in the absence of doxycycline (Tet-Off configuration). The pTRIPZ vector can be easily converted to a Tet-Off configuration by Cre-loxP site-specific recombination or a standard restriction digest. The rtTA3 is flanked by loxP sites allowing in vitro or in vivo excision of the rtTA3 by exposure to Cre recombinase. The rtTA3 is also flanked by a pair of BamHI restriction sites allowing for straightforward cleavage and ligation of the vector to remove the rtTA3. Without the rtTA3 present, a tetracycline transactivator (tTA) can be added extraneously to the system allowing it to function as Tet- Off®; where expression of shRNA and TurboRFP are alternatively induced in the absence of doxycycline. |
Caracteristicas | Inducible shRNA expression for regulatable gene silencing |
Marca | HORIZON |
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