shMIMIC Human Lentiviral microRNA UHT
Disponible
microRNAs, endogenous non-coding ~22mer RNAs, are highly conserved in mammals and regulate gene expression post-transcriptionally through innate RNA interference mechanisms. Now, stable cell lines expressing microRNAs of interest can be created using novel vectors with your choice of promoter and reporter.
Aplicaciones | Silenciamiento génico |
---|---|
Categoría de Producto | microRNA |
Subcategoría de Producto | Mimic |
Temperatura Envío | Congelado -20º |
Temperatura Almacenaje | Congelado -80º |
Disponibilidad | Bajo pedido |
Especie/Huesped | Humano |
Especificaciones | Patented expression scaffold for consistent and correct processing of the mature microRNA Your choice of seven different constitutive promoters to optimize microRNA expression in your cells Design modifications promote preferential loading of the mature microRNA into RISC and robust function High-titer, concentrated lentiviral particles permits delivery into cells refractory to lipid-based delivery Expression of TurboGFP or Turbo RFP (Evrogen, Moscow) allows visualization of transduction efficiency Integrated over-expression of mature microRNA in biologically relevant cell lines advances the long-term study of microRNA-induced gene target modulation and phenotypic effects. microRNA-induced down-regulation of target genes is often subtle, and phenotypes difficult to discern. To ascertain the function of a microRNA, over-expression must be robust and reproducible. Thus, careful attention must be paid to the promoter driving the expression of the microRNA vector. The SMARTchoice platform allows you to customize your shMIMIC microRNA with the promoter and reporter combination that is most suitable for your cells and assays. |
Caracteristicas | Innovative microRNA vector design coupled with your choice of optimal promoter and reporter for over-expression of mature microRNAs |
Marca | HORIZON |
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