Reactivos Expresión Génica -Edit-R CRISPRa Lentiviral hCMV-Blast-dCas9-VPR Plasmid DNA
Disponible
The CRISPR activation (CRISPRa) system is a variation of the canonical CRISPR-Cas9 used for creation of double-strand breaks in genomic DNA. It utilizes a nuclease-deactivated S. pyogenes Cas9 (dCas9), often called "dead Cas9", that is fused to one or more transcriptional activators. When paired with a well-designed guide RNA that targets a gene near a promoter region, the gene's native transcription start site is activated. Edit-R Lentiviral dCas9-VPR Reagents are available with three different promoters (hCMV, mCMV, or hEF1a) and can be supplied as either purified high-titer lentiviral particles or purified plasmid DNA.
Aplicaciones | Expresión génica |
---|---|
Categoría de Producto | Cas9 lentiviral |
Subcategoría de Producto | Lentiviral (CRISPRa) |
Temperatura Envío | Ambiente |
Temperatura Almacenaje | Congelado -20º |
Disponibilidad | Bajo pedido |
Especificaciones | Choose from one of three different promoters for optimum expression in your cells of interest • Provided as concentrated, purified lentiviral particles for immediate transduction, with a minimum ≥ 1 × 107 TU/mL functional titer, by qPCR titering • Also available as certified endotoxin-free plasmid DNA for direct transfection into a packaging cell line and production of your own lentiviral particles • Selection using blasticidin resistance marker (BlastR) for entire cell populations or for clonal expansion of individual cells |
Caracteristicas | Purified lentiviral particles or plasmid DNA for generation of stable dCas9-VPR nuclease-expressing cell populations |
Marca | HORIZON |
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