Reactivos Edición Génica -Edit-R hCMV-PuroR-Cas9 Expression Plasmid DNA
Disponible
Endotoxin-free, purified DNA for direct co-transfection with Edit-R synthetic crRNA and tracrRNA. Choose from three options to facilitate enrichment of Cas9-expressing cells.
Aplicaciones | Edición génica |
---|---|
Categoría de Producto | Cas9 plásmido |
Temperatura Envío | Ambiente |
Temperatura Almacenaje | Congelado -20º |
Disponibilidad | Bajo pedido |
Especificaciones | There are three Edit-R Cas9 Nuclease Expression plasmid options from which to choose, based on preference of enrichment method and cell types: 1.Edit-R Cas9 Expression plasmids with mKate2: Cas9 nuclease and the mKate2 fluorescent reporter are both expressed under the control of a single RNA pol II promoter, making this plasmid useful for downstream cell enrichment by FACS. Offered with your choice of six different promoters. 2.Edit-R Cas9 Expression plasmids with PuroR: Cas9 nuclease and the Puromycin-resistance marker are both expressed under the control of a single RNA pol II promoter, making this plasmid useful for downstream cell enrichment by antibiotic (Puromycin) selection. Offered with your choice of six different promoters. 3.Edit-R Cas9 Nuclease Expression plasmid with hCMV-BlastR: Cas9 nuclease expression is driven from a human cytomegalovirus (hCMV) promoter, and Blasticidin resistance (BlastR) is under the control of the Simian virus 40 (SV40) promoter. This simple vector is useful for those who do not want a fluorescent protein constitutively expressed in the cells of interest and prefer to enrich for Cas9-expressing cells through Blasticidin treatment, especially if a longer antibiotic selection time is required. Not all RNA pol II promoters are equally active in different cellular environments The activity of any given promoter controlling the transcription of Cas9 nuclease can differ greatly from one biological context to another, resulting in variable Cas9 expression levels and thus varying levels of DNA cleavage. Choosing an optimal promoter for your cell line or type will therefore affect the degree of gene editing in your experimentation. The Edit-R Cas9 Nuclease expression plasmids are offered with six different, well-characterized cellular promoters from which you can choose. |
Caracteristicas | Non-lentiviral Cas9 expression plasmids with your choice of promoter and enrichment method |
Marca | HORIZON |
Recomendaciones
-
SMARTvector Non-targeting Control mEF1a-TurboGFP U2.868,95 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Human Inducible Phosphatase Lentiviral6.852,12 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
ON-TARGETplus siRNA Library - Cytokine Receptors - SMARTpool - Mouse4.535,95 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
-
SMARTvector Mouse GAPD Control hEF1a-None Glycerol463,06 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Non-targeting mEF1a-TurboGFP Control P830,71 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
-
SMARTvector Human Epigenetics Lentiviral shRNA Poo9.084,48 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
EGFR Multiplex cfDNA Reference Standard Set596,23 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
-
Controles Edición Génica -Edit-R Lentiviral sgRNA Non-targeting Control #3421,89 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Mouse Inducible DNA Damage Response Le6.852,12 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
siGENOME siRNA RTF - Epigenetics - Mouse11.506,53 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
GIPZ Lentiviral Mouse shRNA Gene Set (glycerol set529,24 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Edit-R Human All-in-one PPIB hEF1a-EGFP Lentiviral sgRNA Positive Control Inventory 50 µl particles552,12 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Non-targeting mEF1a-TurboGFP Glycerol463,06 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Mouse Apoptosis Lentiviral shRNA Poole5.710,95 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
-
Molecular Barcoded Yeast (MoBY) ORF Collection (gl4.422,71 € sin ivaPara ver tus precios y comprar Iniciar sesión