KIT DIAGNÓSTICO ELISA, CYSDV PARA 480 ENSAYOS, 1 KIT
Cucurbit yellow stunting disorder virus Cucurbit yellow stunting disorder virus (genus Crinivirus) was fi rst discovered in the United Arab Emirates (4). (genus Crinivirus) was first discovered in the United Arab Emirates (4). The disorder occurs mainly in areas where the whitefl y vector Bemisia tabaci Bemisia tabaci has been established, e.g., the has been established, e.g., the Arabic Peninsula, Eastern and Western Mediterranean basin, some African countries and North America. CYSDV causes serious damage to cucurbit crops with an approximated yield reduction of 30-50% (1, 4, 5).
Different species of cucurbits (i.e., melons, cucumber, squash and watermelon) are affected, but other hosts such as lettuce, alfalfa, bean and weed species are infected as well but remain symptomless (6). On cucurbits symptoms normally appear as bright yellowing of older leaves. CYSDV symptoms are often confused with nutrient defi ciency symptoms. The phloem-limited virus is neither seed nor mechanically transmitted, but is transmitted with infected propagation material (transplants). CYSDV is on the EPPO A-2 list (3).
Aplicaciones | Diagnóstico vegetal |
---|---|
Categoría de Producto | Test diagnóstico de plantas |
Subcategoría de Producto | ELISA |
Formato Especial | 96 poc. |
Disponibilidad | Bajo pedido |
Patógeno/Antígeno | CYSDV |
Especificaciones | These DAS-ELISA reagents (2) contain polyclonal antibodies made against a recombinant coat protein of CYSDV; the original viral RNA was isolated from infected, greenhouse-grown cucumbers in Lebanon (5). The antibodies have been validated using infected and healthy plants of different cucurbit species (such as cucumber and melon); they are suitable for specifi cally recognizing CYSDV from cucurbits. The virus can be detected in leaves throughout the whole growing season. The highest virus concentration is present in leaf tissues showing early symptoms of interveinal chlorosis, but not yet completely yellow. Since CYSDV is phloem limited, it is preferred to include the leaf mid vein in the sample to be extracted. Samples are homogenized 1:20 (w/v) in extraction buffer «General» (Art. No. 110120). The product was developed in cooperation with the Faculty of Agricultural and Food Sciences, American University of Beirut, Lebanon. |
Marca | BIOREBA |
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