Kit de transfección de lineas celulares SF para Nucleofector HT, formato placas de 384 pocillos, 10 placas

The SF Cell Line 384-well Nucleofector^TM Kit is one of our three kits suited for high-throughput transfection of cell lines when working with the 384-well HT Nucleofector^TM System.

This kit has been determined to be optimal for transfecting following cell types:

• 293-F (FreeStyle), 32D
• A-20, A2058, A375, A431, A549, AGS, ARPE-19
• BetaTC/6, BxPC-3
• C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1
• EL4
• FDC-P1
• H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C
• IMR-32
• J774A.1
• K-562
• KG-1
• L-428, LNCaP
• MDA-MB-453, MDBK, Molt-4, MV-4-11
• Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0
• P19, P815, PC-12, PC-3
• Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226
• S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480
• T2, T84, TF-1
• U266B1, U-937
• Vero

The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.

Benefits

• High transfection efficiencies in difficult-to-transfect cell lines
• Cells maintain functionality post transfection
• Seamless integration into automated liquid handling environments
• Conditions are transferable between 4D-Nucleofector^TM System, 96-well Shuttle^TMSystem and 384-well Nucleofector^TM System

Applications

• Transfection of low cell numbers down to 2 x 10⁴ cell lines
• High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries