Kit de transfección de líneas celulares SF para Nucleofector 4D-LV, kit XL

The SF Cell Line 4D-NucleofectorTM LV Kit XL* is suited for larger scale transfection of cell lines in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit. With the LV NucleocuvetteTM Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.

This kit has been determined to be optimal for transfecting following cell lines and many others that use Solution SF as optimal NucleofectorTM Solution:

• HEK293 suspension clones
• CHO-S suspension clones
• K-562
• U-937

*Note: From the three cell line solutions (SE, SF, SG) that come with the 4D-NucleofectorTM System only SF is available for the large-scale formats so far. Solution SF is suited for the vast majority of cell lines that might be used in larger scale applications (e.g. suspension CHO or 293 cells). For other solutions please contact our Scientific Support.

Benefits

• Closed Nucleofection of up to 109 cells
• Conditions transferable from existing smaller scale protocols
• Automatic processing via tubing system
• Transfection of up to 20 mL reaction volume in less than 10 minutes

Applications

• For large scale transfection of up to 1 x 109 cells
• Transient production of potential therapeutic proteins or antibodies for construct screening
• Generation of large numbers of transiently modified cell lines for cell-based assays