Kit de transfección de lineas celulares SE para Nucleofector HT, formato placas de 384 pocillos, 10 placas

The SE Cell Line 384-well Nucleofector^TM Kit is one of our three kits suited for high-throughput transfection of cell lines when working with the 384-well HT Nucleofector^TM System.

This kit has been determined to be optimal for transfecting following cell types:

• 3T3-L1, diff., 3T3-L1, undiff.
• A-10, A7r5, 
• BHK-21, BJ
• C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
• DU145
• GH3
• HCT116, HeLa, HeLa S3
• IMR-90
• Jurkat (DSMZ), Jurkat E6.1
• KG-1a, L6
• MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
• NCTC clone 929
• Panc-1
• T/G-HA VSMC, T-47D
• U2OS, U-87 MG
• WI-38

The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.

Benefits

• High transfection efficiencies in difficult-to-transfect cell lines
• Cells maintain functionality post transfection
• Seamless integration into automated liquid handling environments
• Conditions are transferable between 4D-Nucleofector^TM System, 96-well Shuttle^TMSystem and 384-well Nucleofector^TM System

Applications

• Transfection of low cell numbers down to 2 x 10⁴ cell lines
• High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries