Kit de transfección de células primarias para Nucleofector 96 pocillos P3 1 kit de 960 reacciones

The P3 Primary Cell 96-well Shuttle^TM Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 96-well Shuttle^TM Add-On.

This kit has been determined to be optimal for transfecting following cell types:

• B cell (human)
• CD34+ cell (human)
• Chondrocyte (human)
• DC (human)
• DC (mouse), mature
• ES (human), H9
• ES (mouse)
• Hepatocyte (human)
• HMEC (human)
• Keratinocyte (human)
• Macrophage (human)
• Monocyte (human)
• Neuron (rat), brain Hi/Cx
• NHBE (human)
• RPE (human)
• T cell (human), stim.
• T cell (mouse), unstim.

The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.

Benefits

• High transfection efficiencies for primary cells
• Compatible with liquid handling systems for automated high-throughput electroporation
• Conditions are transferable between 4D-Nucleofector^TM System, 96-well Shuttle^TMSystem and 384-well Nucleofector^TM System

Applications

• Transfection of low cell numbers in the range of 5 × 10⁴ to 1 × 10⁶ cells
• High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries