Kit de transfección básico de células primarias para parásitos, Kit #2, 1 kit de 25 reacciones

Content

2.25 mL NucleofectorTM Basic Solution for Parasites
0.5 mL Supplement 3
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes

Nucleofector Kits for Parasites:

• Parasitic protozoa infect vertebrates and invertebrates and some are even parasitic in plants.
• In humans they can cause severe diseases such as Malaria (Plasmodium), Sleeping Sickness (Trypanosoma) or Leishmaniasis (Leishmania).
• Nucleofection has proven to provide strongly increased transfection efficiencies (e.g. in Plasmodium berghei and Trypanosoma brucei) compared to standard methods such as electroporation or particle bombardment.
• Due to significant genotypic and phenotypic diversity between species and life cycles Lonza has developed two Basic Parasite Nucleofector Kits (1 and 2) and an easy-to-use Basic Parasite Nucleofector Starter Kit.

Fast determination of ideal Nucleofection Conditions:

• Increased transfection efficiencies (up to 1000 times higher than conventional electroporation),
• Proven performance (successfully used for Plasmodium berghei
• Plasmodium yoelii
• Trypanosoma brucei
• Toxoplasma gondii
• Eimeria tenella
• Babesia bovis
• Leishmania and Perkinsus marinus), 
• Analysis of transient or stable transfections possible