Kit de optimización de transfección de células primarias para Nucleofector HT formato placas de 384 pocillos, 1 placa

The Primary Cell Optimization 384-well Nucleofector^TM Kit is suited for high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector^TM System.

Complete Optimization: Different conditions can easily be tested within one experiment using a 384-well NucleocuvetteTM Plate and our pmaxGFP^TM Vector as positive control. Our five Primary Cell Nucleofector^TM Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. A subsequent optional fine tuning of the Nucleofection conditions can be performed with the help of our scientific support team.

Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector^TM Solutions and Nucleofector^TMPrograms. Basic Protocols are available for following cell groups:

• Endothelial cells (mammalian)
• Epithelial cells (mammalian)
• Fibroblasts (mammalian)
• Glial cells (mammalian)
• Neurons (mammalian)
• Smooth muscle cells (mammalian)
• Stem cells (human)

Benefits

• High transfection efficiencies for primary cells
• Automated high-throughput electroporation
• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
• Optimal Nucleofection conditions determined on the 384-well HT Nucleofector^TMSystem are transferable to the 4D-Nucleofector^TM System or the 96-well ShuttleTMAdd-On

Applications

• Transfection of low cell numbers in the range of 2 x 10⁴ to 1 x 10⁶ cells
• Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector^TM System