Individual SMARTvector Human LNC shRNA Glycerol
Disponible
Guaranteed gene silencing with state-of-the-art shRNA designs and multiple promoter and reporter options.
Aplicaciones | Silenciamiento génico |
---|---|
Categoría de Producto | shRNA SMARTvector |
Subcategoría de Producto | No inducible |
Temperatura Envío | Congelado -20º |
Temperatura Almacenaje | Congelado -80º |
Formato Especial | Individual |
Disponibilidad | Bajo pedido |
Capacidad/Volumen | 2 ml |
Especie/Huesped | Humano |
Especificaciones | SMARTvector Lentiviral shRNA Target any gene in human, mouse and rat, and tailor experiments to specific cells with multiple promoter options Guaranteed silencing (see Specifications tab) Designed using microRNA scaffold-specific attributes for highly efficient processing via the endogenous RNAi pathway Available as glycerol stock and as purified, concentrated lentiviral particles Standard titers of 1 x 108 TU/mL, ± 20%, and ultra-high titers of 5 x 109 TU/mL, ± 20%; functional titers determined by flow cytometric analysis of GFP-positive, transduced HEK293T cells Suitable for dividing and non-dividing cell types, including difficult-to-transfect cells such as primary, neuronal and stem cells Together, these attributes greatly enhance the functionality and specificity of lentiviral-mediated RNAi and reduce the toxicity associated with low titer preparations (supernatant). Superior Dharmacon design algorithm Utilization of a highly processed, patented microRNA scaffold Rationally-designed, highly functional gene targeting sequences Algorithm trained on more than 500 endogenous mRNA data points for functional knockdown testing Incorporation of bioinformatics strategies to reduce off-target gene knockdown events Protein coding genes are targeted in the ORF and 3’UTR; lncRNAs are targeted throughout the entire transcript length Lentiviral particle formats At Dharmacon, we are committed to delivering high quality lentiviral particle preparations. As such, we clone, package and concentrate each construct with strict QC controls. Due to the inherent complexity of lentiviral particle production and the quality control procedures, turnaround time is estimated to be 4 to 6 weeks. We offer the following formats: Set of 3 different gene-targeting constructs Ideal for evaluating multiple shRNAs targeting the same gene and ensuring mRNA knockdown in your specific cells 100 µL (4 x 25 µL) or 200 µL (8 x 25 µL) total amounts at 1 x 108 TU/mL, ± 20%, functional titers determined by flow cytometric analysis of GFP-positive, transduced HEK293T cells Individual gene-targeting constructs Ideal for ordering one or two constructs that you have identified as being the best performers 100 µL (4 x 25 µL) or 200 µL (8 x 25 µL) total amounts at 1 x 108 TU/mL, ± 20%, or 100 µL (4 x 25 µL) total amount at 5 x 109 TU/mL, ± 20%, functional titers determined by flow cytometric analysis of GFP-positive, transduced HEK293T cells SMARTvector Lentiviral Positive and Negative Controls for shRNA and microRNA experiments Ideal for optimizing transduction and assay conditions prior to gene-specific experimentation Negative control particles allow for distinguishing sequence-specific silencing from non-specific effects Positive control particles allow for confirmation of gene silencing using confirmed shRNA constructs targeting a housekeeping gene 50 µL (2 x 25 µL) at 1 x 108 TU/mL, ± 20% or 100 µL (4 x 25 µL) at 5 x 109 TU/mL, ± 20%, functional titers determined by flow cytometric analysis of GFP-positive, transduced HEK293T cells Click here to learn about SMARTvector controls |
Caracteristicas | Make smart, informed choices for successful gene silencing in your cells of interest |
Marca | HORIZON |
Recomendaciones
-
Mouse ON-TARGETplus - RTF Optimization Plates496,59 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
shMIMIC Inducible Rat Lentiviral microRNA 200 µL1.218,95 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Inducible Non-targeting mEF1a-TurboRFP830,71 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Human GPCR Lentiviral shRNA Pooled Lib5.710,95 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
ON-TARGETplus GAPD Control siRNA (Rat)412,77 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Human GAPD mEF1a Control UHT2.868,95 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Non-targeting mEF1a-TurboGFP Control P830,71 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Edit-R POU5F1 CRISPRa crRNA control (Human)218,65 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
-
Reactivos Edición Génica -Edit-R Lentiviral hCMV TurboGFP-Cas9 Nuclease Plasmid DNA351,00 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Inducible Non-targeting mEF1a-TurboRFP463,06 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Mouse DNA Damage Response Lentiviral s5.710,95 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
-
Control #1 sgRNA sintético sin diana Edit-R431,89 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SMARTvector Non-targeting mEF1a-TurboGFP Glycerol463,06 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Individual SMARTvector Mouse Inducible LNC shRNA G489,53 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
siGENOME siRNA Library - Phosphatases - Set of 4 - Mouse9.285,95 € sin ivaPara ver tus precios y comprar Iniciar sesión