Perform specific and efficient, transient or long-term RNAi with GIPZ Lentiviral shRNA. GIPZ Lentiviral shRNA targets human and mouse genes using multiple shRNA constructs that have been cloned into the pGIPZ lentiviral vector.
microRNA-adapted shRNA for specific knockdown
TurboGFP marks cells expressing shRNA
Genome-wide human and mouse representation
Lentiviral delivery extends RNAi to primary and non-dividing cells
Generation and maintenance of stable cell lines facilitated by puromycin selection marker
Pre-designed human and mouse GIPZ shRNA designs are available as bacterial glycerol stocks or high-titer lentiviral particles. Search for your gene(s) of interest to find
Individual shRNA constructs, supplied as E. coli glycerol stocks or high-titer lentiviral particles (1x108 TU/mL, ± 20%, functional titer obtained by flow cytometric analysis of transduced GFP-positive HEK293T cells)
shRNA Starter Kits which include positive and negative controls, plus delivery reagent
Target Gene Sets comprised of 3 to 6 GIPZ shRNA constructs
*One out of three GIPZ Lentiviral shRNA is guaranteed to produce gene knockdown by ≥ 70% at the mRNA level when used in shRNA Starter Kit. Knockdown should be compared to non-silencing control and experimental workflow must be controlled using the provided GIPZ GAPDH positive control.
GIPZ Lentiviral shRNA Starter Kits
The GIPZ Lentiviral shRNA Starter Kits combine the versatility of lentiviral shRNA, the convenience of experimental controls and the necessary mode of delivery for RNAi experimentation.
Your choice of GIPZ lentiviral shRNA constructs targeting human or mouse genes
Positive and negative GIPZ lentiviral shRNA controls
Kits for delivery of GIPZ shRNA by transfection or transduction
Lentiviral particle starter kits provided as 2 x 25 µL aliquots of each gene-targeting construct, 1 x 25 µL aliquots of each control
Guaranteed knockdown when at least 3 GIPZ shRNAs are tested following protocols and using the included GIPZ shRNA positive and negative controls
The GIPZ Transduction Starter Kit includes:
Three GIPZ shRNA as E. coli glycerol stock cultures
Trans-Lentiviral packaging mix sufficient for 10 packaging events
Calcium phosphate transfection reagent
Positive and negative GIPZ lentiviral shRNA controls‡
Guaranteed knockdown† when at least 3 GIPZ shRNAs are tested following protocols and using the included GIPZ shRNA positive and negative controls
The GIPZ Lentiviral shRNA Transfection Starter Kit with DharmaFECT kb includes:
Three GIPZ shRNA as E. coli glycerol stock cultures
Positive and negative GIPZ lentiviral shRNA controls
DharmaFECT transfection reagent for delivery of shRNA plasmid
Guaranteed knockdown† when at least 3 GIPZ shRNAs are tested following protocols and using the included GIPZ shRNA positive and negative controls
Transduction-ready high-titer lentiviral particle format reduces time, labor, and costs involved in prepping DNA, packaging and purifying lentiviral particles for your RNAi experiments. Concentrated, high-titer lentiviral particles allow multiple experiments in a variety of cell types requiring variable multiplicity of infection (MOI). Lentiviral particles are provided at titers of 1x108 TU/mL, ± 20%, functional titer obtained by flow cytometric analysis of transduced GFP-positive HEK293T cells).
***Please note that due to the inherent complexity of high-titer lentiviral particle production, we estimate a 6 to 8 week turnaround time.
† When you purchase a minimum of three shRNA to the same target, at least one of the shRNA constructs will reduce target mRNA levels by 70% or more when used with Starter Kit protocols and normalized for transfection efficiency. Transfection conditions should be confirmed using appropriate positive controls provided in the kit and the percent-knockdown should be compared to cells transfected with the negative control (non-targeting) shRNA.
Please note that the GIPZ Lentiviral shRNA and TRIPZ Inducible Lentiviral shRNA are not compatible with third generation packaging systems, such as ViraPower from Invitrogen. We recommend the Trans-Lentiviral Packaging System to general lentiviral particles for transduction of GIPZ and TRIPZ shRNA.
Caracteristicas
microRNA-adapted shRNA based on miR-30 for specific gene silencing with minimal cytotoxicity
