Fibroblastos pulmonares humanos DHLF-COPD (EPOC)

CloneticsFibroblasts have been isolated from normal, asthma, COPD and cystic fibrosis donors. These are available for variety of research areas including pulmonary, cardiology, dermatology, gastrointestinal and oral research Fibroblasts are found in the stroma of tissue where they play several important roles such as manufacturing growth factors and protein fibers, synthesizing extracellular matrix and collagen. A fibroblast maintains the structural framework for animal tissues and plays a critical role in wound healing. CloneticsFibroblast Growth Media Products are specially designed to support the growth of human fibroblasts. Media systems have been specifically developed and optimized for various cell systems. Media are supplied in several formats: Basal Medium - contains no growth factors, cytokines, or supplements SingleQuotsKit - growth factors, cytokines, and supplements BulletKit- basal medium and SingleQuotsKit packaged together Cloneticsdiseased fibroblasts include lung fibroblasts isolated from adult lung tissue of asthma, COPD, cystic fibrosis and IPF patients. The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, for example, they have long branching processes and gap junctions. Their principle function is production of type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. Pulmonary fibroblasts (PF) play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. For example, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. Important research applications includ pulmonary disease, i.e. COPD, asthma, CF, TB, cancer. Cryopreserved CloneticsDiseased Lung Fibroblast cells stain negative for von Willebrand factor VIII, cytokeratins 18 and 19, and alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Recommended medium: FGM-2 BulletKit Lonza guarantees the performance of Clonetics/Poieticscells only if appropriate Clonetics/Poieticsmedia and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support. Note: Learn more about our additional products for airway research. Note: Contact Scientific Support for detailed donor information including age of diagnosis, medications taken, and smoking history.