Edit-R Pooled sgRNA Indexing PCR and Sequencing Pr
Disponible
Fully sequenced pooled libraries of potent, algorithm-designed lentiviral sgRNA demonstrating efficient gene editing at single-copy integrations.
Aplicaciones | Edición génica |
---|---|
Categoría de Producto | Librerías CRISPR |
Subcategoría de Producto | Cas9/sgRNA |
Temperatura Envío | Congelado -20º |
Temperatura Almacenaje | Congelado -20º |
Disponibilidad | Bajo pedido |
Especificaciones | Efficient two-vector system that utilizes a lentiviral vector for Cas9 expression and a gene-specific vector for sgRNA expression Rationally designed Edit-R lentiviral sgRNAs efficient gene knockout with unparalleled specificity using a functionally validated proprietary algorithm Deep and broad coverage of 5 -10 sgRNAs per gene across the human, mouse and rat genomes for increased hit confidence and comprehensive genome screening Can be combined with the promoter flexibility of Edit-R Lentiviral Cas9 Nuclease Reagents for robust editing in biologically relevant cell types Each Edit-R Lentiviral sgRNA Pooled Screening Library includes ≥ 5 x 108 TU/mL ( ± 20%) lentiviral particles in pre-aliquoted tubes 8 x 25 µL (200 µL total) for libraries ≤ 5000 constructs 16 x 25 µL (400 µL total) for libraries > 5000 constructs Five to ten sgRNAs per gene targeting coding genes in the NCBI Reference Sequence Database 100 non-targeting sgRNA negative controls bioinformatically confirmed to not align with (target) any gene in the human, mouse and rat genomes. Up to 340 gene-specific sgRNA positive controls targeting up to 34 protein-coding genes (10 sgRNA per gene); including common reference genes (ACTB, GAPDH, LAMB1, & PPIB) A data file containing complete library information, including: gene annotations, sgRNA target sequences, complete list of controls, and counts per millions of mapped reads Edit-R Lentiviral sgRNA libraries are available for defined sub-libraries of gene families up to the entire genome for human and mouse; rat libraries and custom Edit-R Lentiviral sgRNA collections are available upon request. Request a custom collection of human, mouse, or rat gene-targeting sgRNAs Available in pool sizes between 50 to 10,000 constructs Pools are provided as purified, concentrated lentiviral particles ≥ 5 x 108 TU/mL |
Caracteristicas | Whole genome, druggable genome, gene family and pathway lentiviral sgRNA pooled libraries for knockout screening. |
Marca | HORIZON |
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