Células precursoras de osteoclastos
Disponible
The maintenance and repair of bone tissue is primarily attributed to two cell types: osteoblasts which form new bone tissue and osteoclasts which re-absorb bone tissue. Defects in the bone mineralization/bone degradation cycle contribute to a number of bone related diseases such as osteoporosis. Osteoclasts are large, multinucleated cells that play an active role in bone resorption. The PoieticsOsteoclast Precursor Cell system has been designed for use in high-throughput applications to conduct research on osteoporosis, bone resorption, and other bone-related diseases.
Monocytes from the marrow or the blood serve as precursors of osteoclasts. Traditionally, researchers drive populations of purified monocytes to become osteoclasts, however, in some cases, this can result in a differentiation efficiency of as low as 10%. In contrast, Lonza's Osteoclast Precursor Cell System yields up to 50% conversion of precursors to osteoclasts when differentiated.
Cryopreserved PoieticsHuman Osteoclast Precursor Cells (OCPs) are guaranteed to contain ≥1 million viable cells per ampoule and test positive for osteoclast activity and stain for TRAP following differentiation. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Recommended Medium: OCP Octeoclast Precursor BulletKitMedium (provides both negative control/experimental medium which can be used as a negative control [without the addition of additional supplements] or an experimental medium [with the addition of researcher provided differentiation supplements] and a positive control medium guaranteed to induce differentiation into mature, functionally active osteoclasts; Each bottle of the OCP BulletKitMedium contains enough differentiation medium to fully differentiate approximately 1.5 million osteoclast precursor cells [approximately 1.5 complete 96-well plates] into mature, functionally active osteoclasts).
The OCP Medium is offered as a BulletKitMedium (catalog no. PT-8001) which includes both the basal medium and the necessary supplements for both plating and differentiation of osteoclast precursors. The basal medium (OCP Osteoclast Precur. Basal Medium; catalog no. PT-8201) and the necessary supplements (OCP Osteoclast Precur. SingleQuot Kit; catalog no. PT-9501) can also be purchased individually.
Lonza guarantees the performance of Clonetics/Poieticscells only if appropriate Clonetics/Poieticsmedia and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
Aplicaciones | Bioprocesos |
---|---|
Categoría de Producto | Células madre |
Temperatura Envío | Nitrógeno líquido -180º |
Temperatura Almacenaje | Nitrógeno líquido -180º |
Disponibilidad | Bajo pedido |
Capacidad/Volumen | ≥ 1 millón de células |
Especie/Huesped | Humano |
Tipo Celular | Osteoclastos |
Marca | LONZA |
Recomendaciones
-
-
Marcador de peso molecular de ADN, 20 bp para 100 geles, 1 vial 100 µl236,74 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Células madre derivadas de tejido adiposo humano con Diabetees Tipo II, criopreservadas997,99 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Kit de transfección de macrófagos humanos para Nucleofector 2b, 1 kit de 10 reacciones736,73 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Suplementos y factores de crecimiento de medio BEGM para Células del epitelio, bronquial, SingleQuots, 1 kit260,36 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Adult Keratinocyte Cell Culture Kit1.398,77 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Marcador de peso molecular de ADN para cuantificación FlashGel 100 - 1500 pb, 1 vial de 250 µl311,22 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Células de músculo liso aórtico de rata criopreservadas, 4 millones de céls.1.672,45 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Kit de transfección de células primarias humanas AoSMC para Nucleofector 2b, 1 kit de 25 reacciones736,73 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
-
-
Reactivo de electroforesis para tinción de geles de proteínas SYPRO Ruby, 1 botella de 1 l674,88 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Neuronas del ganglio de la raíz dorsal neonatal embrionarias de rata (DRG)2.484,92 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Kit de transfección de cardiomiocitos de rata neonata para Nucleofector 2b, 1 kit de 25 reacciones736,73 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
SFM - Medio especial neuronas primarias de rata PNGM BulletKit, contiene medio basal y suplementos, 1 kit408,50 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
-
Reactivo para biología molecular AccuGENE TAE 10X, 1 botella de 1 l111,33 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Hepatocitos de rata (Wistar) criopreservados, suspensión438,80 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Kit de transfección inicial de células madre humanas para Nucleofector 2b, 1 kit de 18 reacciones736,73 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Solución de disociación tripisina-EDTA, 1 botella de 100 ml90,16 € sin ivaPara ver tus precios y comprar Iniciar sesión