Células madre mesenquimales criopreservadas (hMSC)

PoieticsNormal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) are isolated from normal (non-diabetic) adult human bone marrow withdrawn from bilateral punctures of the posterior iliac crests of normal volunteers. Normal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) have been reported to differentiate down many different lineages including chondrogenic, osteogenic, adipogenic and neural. Normal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) have been cryopreserved after the second passage and are suggested to be used by no later than the fifth passage. Cryopreserved PoieticsNormal Human Bone Marrow Derived Mesenchymal Stem Cells (hMSC) are guaranteed to have at least 0.75 million viable cells per vial, differentiate down the adipogenic, chondrogenic, and osteogenic liniages when cultured in the recommended differentiation medium, to express CD29, CD44, CD105, and CD166 and to not express CD14, CD34, and CD45. All lots of this product manufactured after January 1, 2015 are also guaranteed to express CD90 and CD73 and to not express HLA-DR or CD19. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Recommended Growth Medium: MSCGM human Mesenchymal Stem Cell Growth BulletKitMedium (serum containing) or TheraPEAKMSCGM-CDMesenchymal Stem Cell Chemically Defined Medium (serum free) Recommended Differentiation Media: Adipogenic: hMSC Adipogenic Differentiation BulletKitMedium provides both an induction medium and a maintenance medium guaranteed to induce adipogenic differentiation of human bone marrow derived mesenchymal stem cells into mature, functionally active adipocytes; Each bottle of the hMSC Adipogenic Differentiation BulletKitMedium contains enough induction medium and maintenance medium to fully differentiate approximately 3 million human bone marrow derived mesenchymal stem cells [approximately 15 wells in a 6-well plate format] into mature, functionally active adipocytes. Chondrogenic: hMSC Chondrogenic Differentiation BulletKitMedium, with the addition of TGF-ß3 (provides a differentiation medium appropriate to induce chondrogenic differentiation of human adipose derived mesenchymal stem cells into mature, functionally active chondrocytes; Each bottle of the hMSC Chondrogenic Differentiation BulletKitMedium, with the addition of TGF-ß3 (sold separately), contains enough differentiation medium to fully differentiate approximately 12.5 million human adipose derived mesenchymal stem cells [approximately 25 micropellets] into mature, functionally active chondrocytes). Osteogenic: hMSC Osteogenic Differentiation BulletKitMedium (provides a differentiation medium appropriate to induce osteogenic differentiation of human adipose derived mesenchymal stem cells into mature, functionally active osteoblasts; Each bottle of the hMSC Osteogenic Differentiation BulletKitMedium contains enough differentiation medium to fully differentiate approximately 200,000 human adipose derived mesenchymal stem cells [approximately 20 wells in a 24-well plate format] into mature, functionally active osteoblasts). Lonza guarantees the performance of Clonetics/Poieticscells only if appropriate Clonetics/Poieticsmedia and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.