Células dendríticas NHDC criopreservadas

Dendritic Cells (DCs) act as the messenger cells of the immune system by processing antigens from pathogens and presenting the antigens to T cells to initiate an immune response. PoieticsNormal Human Dendritic Cells are tested for a panel of specific surface markers, including CD11c+, CD86+, CD80+. HLADR+, and CD14- to provide detailed characterization of the cells. These cells can survive up to 7 days in culture with the proper cytokines (IL-4 and GM-CSF) to maintain the cells as immature dendritic cells (iDCs). Cells can be further differentiated to mature dendritic cells with the addition of TNF-α and IL-1β. All dendritic cells are differentiated from peripheral blood monocytes isolated via Ficoll density gradient separation and are quality tested for optimal performance. Lonza's Normal Human Dendritic Cells would typically be classified as Mo-DC/MDDC (monocyte derived dendritic cells) or CD11c+ myeloid Dendritic Cells (DC). Recommended Medium: LGM-3Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4 Lonza guarantees the performance of Clonetics/Poieticscells only if appropriate Clonetics/Poieticsmedia and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.