Células B CD19+ (selección negativa), > 10 millón de céls.

Leukapheresed CD19+ B cells are negatively selected from leukapheresed peripheral blood MNCs using an immunomagnetic labeling system to remove unwanted cell types leaving the B cells untouched. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols.

• Generating antibody libraries
• Co-culture with T helper cells or cytotoxic lymphocytes (CTL)
• B cell activation, clonal expansion, Ig rearrangement, and Ig secretion
• Antigen presenting cell (APC) signal transduction and antigen presentation