3-8_ Crit XT Tris-Acetate Gel 18W 30µl

Choose this versatile midi Criterion XT Tris-Acetate Gel for denaturing or nondenaturing PAGE. The gels are made without SDS, which allow the sample buffer and running buffer to determine the separation mechanism.

• For native PAGE use Native Sample and Tris/Glycine Native Running Buffers
• For separation by molecular weight, use XT Sample and XT Tricine Running Buffers
• Neutral pH (pH 7.0) delays acrylamide hydrolysis as compared to traditional Laemmli systems
• 8-month shelf life
• Separation range is ~40–400 kD under denaturing conditions

Under denaturing conditions, with the SDS-containing Tricine running buffer, the discontinuous acetate and Tricine ion fronts form moving boundaries to stack and then separate proteins by molecular weight. During native electrophoresis, where proteins are separated by mass-to-charge ratio, discontinuous acetate and glycine ion fronts form moving boundaries to stack and separate proteins by both size and charge.