Controles Silenciamiento Génico -SMARTvector Non-targeting Control hEF1a-TurboGFP UHT 10

Disponible
The SMARTvector RNAi Controls are cost-effective tools for determining optimal conditions for delivery prior to using gene-specific SMARTvector Lentiviral shRNAs and shMIMIC Lentiviral microRNAs.
Aplicaciones | Silenciamiento génico |
---|---|
Categoría de Producto | Controles shRNA |
Subcategoría de Producto | Negativo |
Temperatura Envío | Congelado -80º |
Temperatura Almacenaje | Congelado -80º |
Disponibilidad | Bajo pedido |
Ref Dutscher | 77VSC11044 |
Especificaciones | Start by identifying the optimal vector configuration for your cells with the SMARTchoice Promoter Selection Plate Determine the most active promoter in the specific cells of interest by evaluating all seven vectors simultaneously using the SMARTchoice Promoter Selection Plate (catalog # SP-001000-01): An arrayed plate of SMARTvector Non-targeting Controls (NTCs) with seven well-characterized constitutive cellular promoters driving the expression of TurboGFP (Evrogen, Moscow, Russia) High-titer lentiviral particles are packaged, purified, and concentrated for consistent activity Lentiviral particles are arrayed in a convenient 96- well format in duplicate serial dilutions so multiple MOIs can be tested. Order SMARTvector RNAi controls with promoter and reporter selections matching your gene-specific constructs. SMARTvector vector-matched controls are available with options for all seven promoters and two fluorescent reporters or without a fluorescent reporter. All individual controls are available as glycerol stocks and as 50 µL (2 x 25 µL) of 1 x 108 TU/mL, ± 20%, and 100 µL (4 x 25 µL) of 5 x 109 TU/mL, ± 20%, of purified, concentrated packaged lentiviral particles. SMARTvector Non-targeting Controls Validated to have minimal targeting of any known genes in the human, mouse and rat genomes Confirm transduction efficiency via TurboGFP or TurboRFP expression Distinguish sequence-specific silencing of gene-targeting SMARTvector shRNAs and shMIMIC microRNAs from non-specific effects Experimental considerations: SMARTvector Non-targeting Control 1: Our longest standing and most commonly used SMARTvector non-targeting control, ideal for supporting most experiments involving only a few genes SMARTvector Non-targeting Control 2 – 12: Designed specifically with the needs of screeners in mind, who may require using several different non-targeting control sequences in follow-up experiments; should be selected if multiple controls are desired SMARTvector Positve Controls Provide consistent, potent silencing of non-essential, abundantly expressed housekeeping genes in human (see Figure 2), mouse and rat cells SMARTvector GAPD Positive Control shRNA (human, mouse and rat); validated to target glyceraldehyde-3-phosphate dehydrogenase (also known as GAPDH) SMARTvector PPIB Positive Control shRNA (human, mouse and rat); validated to target peptidylprolyl isomerase B (also known as cyclophilin B) |
Caracteristicas | Matched Controls enable accurate data interpretation for both SMARTvector Inducible shRNA and shMIMIC Inducible microRNAs |
Marca | HORIZON |
Recomendaciones
-
Reactivos Transfección -DharmaFECT 1 Transfection Reagent573,35 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting Control mCMV-None UHT 72.932,94 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Librería Silenc. Génico SMARTvector Inducible Membrane Trafficking Lentiviral shRNA Pooled Library hEF1a-GFP, Human6.849,12 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Librería Silenciamiento Génico -ON-TARGETplus siRNA Library - Ubiquitin Conjugation Subset 2 - Set of 4 - Human6.583,35 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Controles Silenciamiento Génico -Accell GAPD siRNA - Rat201,00 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Controles Silenciamiento Génico -SMARTvector GAPD Control hCMV-TurboRFP Glycerol - Rat527,06 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting mCMV-None Control Particles 10894,71 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Librería Silenciamiento Génico -SMARTvector Ion Channel Lentiviral shRNA Pooled Library mCMV-RFP - Human5.707,94 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Librería Silenciamiento Génico -ON-TARGETplus siRNA Library - DNA Damage Response - SMARTpool - Human6.721,59 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Controles Silenciamiento Génico -Mimic Transfection Control with Cy3762,76 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Controles Silenciamiento Génico -SMARTvector GAPD Control hEF1a-None Glycerol - Human527,06 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Librería Silenc. Génico SMARTvector Inducible Membrane Trafficking Lentiviral shRNA Pooled Library mEF1a, RFP, Mouse6.849,12 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Controles Silenciamiento Génico -SMARTvector GAPD mEF1a-TurboGFP Control Particles - Rat894,71 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Estándar de referencia KRAS G12D 50_ (formato gDNA) 50ng_µl, 1µg161,66 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting hEF1a-None Glycerol 12527,06 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Librería Silenciamiento Génico -SMARTvector Druggable Lentiviral shRNA Pooled Library hEF1a - Mouse-RFP - Mouse12.452,06 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Reactivos Transfección -DharmaFECT Kb Transfection Reagent1.230,71 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Librería Expresión Génica -MGC Fully Sequenced Human cDNA (pCMV) Set Phosphatase (glycerol stock)1.353,53 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Control positivo sgRNA sintético humano PPIB Edit-R214,24 € sin ivaPara ver tus precios y comprar Iniciar sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting Control mCMV-TurboRFP UHT 82.932,94 € sin ivaPara ver tus precios y comprar Iniciar sesión